FectinMore™ Transfection Reagent
Catalog# Size Price Availability
CM001-1.5T 1.5 mL $188.00
CM001-7.5T 5*1.5 mL $750.00
Product Specifications
Background FectinMore™ Transfection Reagent is a low cell toxicity and non-liposome reagent optimized for DNA delivery. It provides effectively, reproducibly, and affordable benefits for scientific research.
Stability & Storage -20℃
Shipping Blue ice
Product Note

(1) Cell preparation: Cells should be seeded before 16 to 20 hours prior to transfection with around 70% confluence. The medium should be refreshed 30 minutes before transfection. Usually, culture medium with serum does not affect transfection.
(2) DNA preparation: DNA plasmid for transfection should be with high purity (A260/A280=1.8-1.9) to ensure efficient transfection mixture preparation.
(3) Mixture preparation: Guideline of DNA plasmid and FectinMore™ DNA Transfection Reagent amount and ratio refer to table 1. In brief, plasmid and transfection reagent are diluted in serum-free culture medium for 5 minutes then mixed together and tip gently for another 25 minutes.
(4) Transfection: Add mixtures into cell culture dish/plate. The mixture could be removed after 6 to 48 hours and refilled with culture medium.
Recommended formula of transfection mixture:

Culture Dish/Plate Medium Volume Plasmid / Serum-free medium

FectinMore™ DNA Transfection Reagent / Serum-free medium


100 μ L

250 ng/10 μ L

0.75 μ L/10 μL


500 μ L

500 ng/25 μ L

1.5 μ L/25 μ L


700 μ L

750 ng/35 μ L

2.25 μ L/35 μ L


1 m L

1 μg/50 μ L

3 μ L/50 μL

60mm dish

3 m L

2. 5 μg/150 μ L

7. 5 μ L/150 μL

100mm dish

6 m L

5 μg/300 μ L

15 μ L/300 μL


Important notes

FectinMore™ DNA Transfection Reagent is recommended for use with Gibco™ Opti-MEM™ I Reduced-Serum Medium or Gibco™ OptiPRO™ SFM for alternative optimizing transfection efficiency.

Scientific Data

· Transfected with FectinMore™(  FectinMore™:DNA=3:1) ,detect the expression of target protein after 2 days.

Proteins expression checked by western blot in specific primary antibody followed by secondary antibody (anti-Mouse IgG(H+L)- HRP).

Proteins expression checked by FACS in specific primary antibody followed by secondary antibody (anti - Mouse IgG(H +L)- FAM).

Proteins expression checked by immunofluorescence (IFA) in specific primary antibody followed by secondary antibody (anti - Mouse IgG -iFluor 594).

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