Transfection is a widely used laboratory cell culture technique that introduces foreign nucleic acids into cells. It is a powerful analytical tool enabling study of gene functions and protein expressiong in a wide variety of cell types.
Nowadays, various types of nucleic acid can be transfected into mammalian cells including: deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs) as well as small, non-coding RNAs such as siRNA, shRNA, and miRNA.
There are multiple methods for transfection of mammalian cells, which can be subdivided into biological, chemical, and physical methods.
| Advantages and Disadvantages of Different Transfection Methods | |||
| Class | Method | Pros | Cons |
| Chemical | ● Cationic polymer ● Cationic lipid ● Calcium phosphate |
– Relative ease of use – Relatively inexpensive |
– Variable transfection efficiency – Toxicity to cells by some chemical compounds – May be difficult to transfect particular cell types e.g., small cells, nonadherent cells |
| Physical | ● Direct injection ● Biolistic particle delivery ● Electroporation ● Laserfection |
– Can be used for hard-to-transfect cells – May allow single cell transfection – May allow precise insertion of nucleic acid into subcellular compartment |
– May cause significant toxicity to cells – May require specialized equipment and operator skill |
| Biological | ● Virus-mediated | – High transfection efficiency – Can be used for difficult to ransfect cells |
– Requires biosafety standard laboratory space – Risk of insertional mutagenesis – DNA insert size limit |
(From: Sheikh, Semira (2017). Basic Science Methods for Clinical Researchers || Transfection. Page 191–209. doi:10.1016/B978-0-12-803077-6.00011-4)
FectinMore™ Transfection Reagent is a low cell toxicity and non-liposome Cationic polymer reagent optimized for DNA delivery. It provides effectively, reproducibly, and affordable benefits for scientific research.
Product features:
| · Optinized for DNA | · Lower toxicity, Higher cell viability |
| · Better transfection efficiency | · Suitable for transient transfection and stable transfection |
| · Suitable for various cell lines | · Easy to handle |
| · Cost-effective |
| Catalog# | Size | Price | Availability |
| CM001-1.5T | 1.5 mL | $188.00 | |
| CM001-7.5T | 5*1.5 mL | $750.00 |
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