Transfection Reagent

Transfection is a widely used laboratory cell culture technique that introduces foreign nucleic acids into cells. It is a powerful analytical tool enabling study of gene functions and protein expressiong in a wide variety of cell types.

Nowadays, various types of nucleic acid can be transfected into mammalian cells including: deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs) as well as small, non-coding RNAs such as siRNA, shRNA, and miRNA.

There are multiple methods for transfection of mammalian cells, which can be subdivided into biological, chemical, and physical methods.

Advantages and Disadvantages of Different Transfection Methods
Class Method Pros Cons
Chemical ● Cationic polymer
● Cationic lipid
● Calcium phosphate
– Relative ease of use
– Relatively inexpensive
– Variable transfection efficiency
– Toxicity to cells by some chemical compounds
– May be difficult to transfect particular cell types e.g., small cells, nonadherent cells
Physical ● Direct injection
● Biolistic particle delivery
● Electroporation
● Laserfection
– Can be used for hard-to-transfect cells
– May allow single cell transfection
– May allow precise insertion of nucleic acid into subcellular compartment
– May cause significant toxicity to cells
– May require specialized equipment and operator skill
Biological ● Virus-mediated – High transfection efficiency
– Can be used for difficult to ransfect cells
– Requires biosafety standard laboratory space
– Risk of insertional mutagenesis
– DNA insert size limit

(From: Sheikh, Semira (2017). Basic Science Methods for Clinical Researchers || Transfection. Page 191–209. doi:10.1016/B978-0-12-803077-6.00011-4)


FectinMore™ Transfection Reagent is a low cell toxicity and non-liposome Cationic polymer reagent optimized for DNA delivery. It provides effectively, reproducibly, and affordable benefits for scientific research.


Product features:

· Optinized for DNA · Lower toxicity, Higher cell viability
· Better transfection efficiency · Suitable for transient transfection and stable transfection
· Suitable for various cell lines · Easy to handle
· Cost-effective  
Catalog# Size Price Availability
CM001-1.5T 1.5 mL $188.00
CM001-7.5T 5*1.5 mL $750.00