Protein Expression and Purification (Mammalian Cell System)

The mammalian cell expression system is the preferred expression platform for producing mammalian proteins with the most native structure and activity. Therefore, it is an ideal choice for expressing proteins that needs fully post-translational modifications and following applications, such as recombinant antibodies and therapeutic proteins development. 

Key service features:
• Using Leadgene’s exclusive protein expression technique, the expression success rate of the target recombinant proteins can increase to above 90%.
• Flexibility for clients’ requirements, and the small scale production could be executed before large scale production.
• We also provide other additional services such as endotoxin detection, bio-activity analytic platform, tag protein removal, protein subpackage and lyophilization.
• One-stop service from codon analysis to protein purification.

Service workflow:
• Clients provide sequence and protein information of interested target.
• Choose the suitable vector and host system for protein expression.
• Gene synthesis and plasmid production.
• Plasmid transfection into suitable strains of mammalian cell.
• Optimize the condition for protein expression.
• Protein purification.
• Provide purified target protein with analysis report.

Sample requirements:
• Target protein sequence
• Requirement of target protein (including purity, modification, amount and so on)

Deliverables:
• Purified protein and final report (including SDS-PAGE, Western blot and so on)

Protein Expression and Purification (E. coli System)

Based on the clear genetic background and short turnaround time, E. coli-expression system is now the most common-used platform for protein expression. E. coli protein expression system is suitable for expression of multiple species proteins, which serve as an important tool for recombinant protein production and other biological researches.

Key service features:
• Using Leadgene’s exclusive protein expression technique, we are able to raise successful protein expression rate to above 98%.
• Flexibility for client requirement, and the small scale production could be executed before large scale production.
• Experienced in resolving of common problem, such as insoluble protein or low yield of production.
• We also provide other additional services, such as endotoxin detection, bio-activity analytic platform, tag protein removal, protein subpackage and lyophilization.
• One-step service from codon optimization to protein purification.

Service workflow:
• The sequence and protein information of interested targets should be provided.
• Choose the suitable vector and host system for protein expression.
• Gene synthesis and plasmid production.
• Plasmid transfection into suitable strains of E. coli.
• Optimize the condition for protein expression.
• Protein purification
• Provide purified target protein with analysis report.

Sample requirements:
• Target protein sequence
• Requirement of target protein (including purity, modification, amount and so on).

Deliverables:
• Purified protein and final report (including SDS-PAGE, Western blot and so on).