Mouse Polyclonal Antibody Development

Key service features:
• Our experts will offer professional analysis and suggestion for antigen design.
• Our capable project manager and scientists will support and discuss with you personally throughout the process.
• Flexibility to increase the number of immunized animals.
• We provide professional affinity purification technology to increase the purity of antibody.

Service workflow:
• Antigen design and evaluation
• Carrier protein-conjugated peptide synthesis or customized protein production (depend on clients’ requirements).
• Four mouse immunization with your target antigen.
• Collecting the serum from each of antigen-immunized mouse.
• Improving the specificity and affinity of antibody by affinity purification.
• Binding ability test of antibody to immunogen by Indirect ELISA test and QC report.

Sample requirements:
• Clients should offer the sequence for their target peptide or protein immunogen.
• If clients prefer to use their own peptide as immunogen, the following condition should be complied.
Peptide: concentration ≧ 1 mg/mL, Amount ≧ 5 mg, Purity > 85%.
* To confirm the peptide quality, client should provide the HPLC result, MS data and sequence of peptide)
* For client who want to do affinity purification, more than 5 mg of peptide should be provided. (usually needs about 8-10 mg).
• If clients prefer to use their own protein as immunogen, the following condition should be complied.
Protein: concentration ≧ 1 mg/mL, Amount ≧ 3~5 mg, Purity > 90%
* To confirm the protein quality, client should provide the SDS-PAGE, QC data.
* For client who want to do affinity purification, more than 10 mg of protein should be provided.

Deliverables:
• Post-immunized serum.
• Indirect ELISA test of antibody to immunogen and QC report of purified antibody with or without immunization, titer > 1:50,000.

Rabbit Polyclonal Antibody Development

Key service features:
• Our experts will offer professional analysis and suggestion for antigen design.
• Our capable project manager and scientists will support and discuss with you throughout the process.
• Flexibility to increase the number of immunized animals.
• We provide professional affinity purification technique to increase the purity of antibodies.

Service workflow:
• Antigen design and evaluation
• Carrier protein-conjugated peptide synthesis or customized protein production (depending on clients’ requirements)
• Two New Zealand Rabbit immunization with your target antigen
• Collecting the serum from each of antigen-immunized rabbit
• Improving the specificity and affinity of antibodies by affinity purification
• Binding ability test of antibody to immunogen by indirect ELISA test and QC report

Sample requirements:
• Clients should offer the sequence for their target peptide or protein immunogen.
• If clients prefer to use their own peptides as immunogens, the following condition should have complied.
Peptide: concentration ≧ 1 mg/mL, Amount ≧ 5 mg, Purity > 85%.
* To confirm the peptide quality, the client should provide the HPLC result, MS data, and sequence of the peptides.
* If the clients want to do an affinity purification, they need to provide at least 5 mg of peptides. (We usually need 8-10 mg.)
• If clients prefer to use their own proteins as immunogens, the following condition should have complied.
Protein: concentration ≧ 1 mg/mL, Amount ≧ 3~5 mg, Purity > 90%
* To confirm the protein quality, the client should provide the SDS-PAGE, QC data.
* If the clients want to do an affinity purification, they need to provide at least 10 mg of proteins.

Deliverables:
• 1 mg peptide or X mg protein immunogen (depend on client requirement)
• 0.5 mL pre-immunized serum/rabbit (If not need purification)
• 35 mL post-immunized serum/rabbit (If not need purification)
• 50 μg Control IgG antibody
• 2-10 mg purified rabbit polyclonal antibody
• Indirect ELISA test of antibody to the immunogen and QC report of purified antibody with or without immunization, titer > 1:50,000.