R-spondin 1 (RSPO1, Roof plate-specific Spondin 1) is a cysteine-rich secreted glycoprotein that belongs to the R-Spondin family. Structurally, R-spondin 1 harbors an N-terminal signal peptide, two cysteine-rich furin-like (FU1-FU2) domains, a thrombospondin (TSP) domain, and a basic amino acid-rich (BR) C-terminal domain. R-spondin 1 acts as a ligand for the leucine-rich repeat-containing G-protein coupled receptor (LGR) family (LGR4/5/ 6), subsequent clearing negative regulators ZNRF3 /RNF43 from the membrane, leading to the availability of Wnt receptor and activating Wnt/β-catenin signaling. The signal cascade is essential for regulating stem cell proliferation, embryonic development, and tissue homeostasis.

CHAMOT can provide Recombinant Human R-Spondin 1, His-SUMO tag, HEK293 with high activity, low endotoxicity, high purity and no animal origin components to facilitate organoid research.

Product performance:

Expression System: HEK293 cell

Activity: R-Spondin-1 enhances BMP-2-mediated differentiation of MC3T3-E1 cells. The ED₅₀ for this effect is 1-3 μg/mL. Activity: R-Spondin-1 enhances BMP-2-mediated differentiation of Mc3t3-e1 cells. The Ed ₅₀ for this effect is 1-3 μg/mL.

Purity: >95% as determined by SDS-PAGE analysis.

Endotoxin Level: <1 EU per 1 μg of the protein by the LAL method.

SDS-PAGE analysis of recombinant human R-Spondin 1.