Q: Are CHAMOT's cytokines lyophilized?

A: CHAMOT's cytokines are all in the form of lyophilized powder, which is more conducive to the transportation and stability of the product.

Q: Are CHAMOT's cytokines sterile?

A: CHAMOT's cytokines are filtered and sterilized before lyophilization. Simply dissolve in sterile water or sterilized buffer according to the manual.

Q: How to convert ED50 and units/mg?

A: ED50 is defined as  the concentration of the cytokines that induces 50% of the maximum response. Testing of the ED50 varies from company to company. The formula to convert ED50 in ng/mL to specific activity (in units/mg) is：

 Specific activity(units/mg)=1×10^6/ED50(ng/ml)

Q: How to compare the cross reactivity of cytokines?

A: Blast tool: "http://www.ebi.ac.uk/blastall/"

Find the amino acid sequence of the protein from the product manual, paste it into the comparison box, and click "Submit" to start the comparison. According to the Identity% of the result, the cross-reactivity of the desired species was judged (generally more than 85% will be thought to have theoretical cross-reactivity).

Q: What is Animal-free recombinant protein?

A: The recombinant proteins provided by CHAMOT are all Animal-free grade, where Animal-free refers to the process of expressing the protein without the use of any animal-derived materials. Normally animal-derived components such as tryptone need to be added into the medium when using E. coli expression system, while these animal-derived components of alloproteins may cause xenorejection or allergic reactions in humans and animals in the future.

CHAMOT recombinant protein is generally expressed by Escherichia coli, while these proteins are produced in independent animal-free production facilities without the use of culture-medium or reagents containing animal-derived ingredients such as tryptone.